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versadoc bio rad imaging system  (Bio-Rad)


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    Structured Review

    Bio-Rad versadoc bio rad imaging system
    Versadoc Bio Rad Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 34215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+versadoc+imaging+system/ChemiDoc+Imaging+System/pm40472778-81-11-12
    Average 99 stars, based on 34215 article reviews
    versadoc bio rad imaging system - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: The O 6 -Methyguanine-DNA Methyltransferase Inhibitor O 6 -Benzylguanine Enhanced Activity of Temozolomide + Irinotecan Against Models of High-Risk Neuroblastoma
    Article Snippet: Pellets were extracted in RIPA buffer (Pierce) with 1 mM sodium orthovanadate, 1 mM sodium fluoride, and protease inhibitor cocktail (Set III, Calbiochem). .. Cleaved caspase-3 and p-gamma-H2AX were assessed by immunoblot and imaged on a Bio-Rad VersaDoc imaging system (CHLA-225, COG-N-269, and COG-N-452h) or using Amersham Hyperfilm ECL (COG-N-561h). .. Anti-cleaved caspase-3 (Cell Signaling, 9664S) and anti-phosphoserine-139 gamma-H2AX (Novus, NB100–384) antibodies were diluted 1:1000.

    Article Title: CD38–RyR2 axis–mediated signaling impedes CD8 + T cell response to anti-PD1 therapy in cancer
    Article Snippet: Concurrently, pre-stained protein markers (Invitrogen, Thermo Fisher Scientific, Waltham, MA) were run in parallel to identify the molecular weight of the detected proteins. .. Chemiluminescent detection with Clarity Western ECL substrate (Bio-Rad, Hercules, CA) was visualized on Bio-Rad Versadoc Imaging System. .. The same membrane was then stripped and reprobed with β-actin antibody (Cell Signaling Technologies, Danvers, MA) for normalization.

    Imaging:

    Article Title: The O 6 -Methyguanine-DNA Methyltransferase Inhibitor O 6 -Benzylguanine Enhanced Activity of Temozolomide + Irinotecan Against Models of High-Risk Neuroblastoma
    Article Snippet: Pellets were extracted in RIPA buffer (Pierce) with 1 mM sodium orthovanadate, 1 mM sodium fluoride, and protease inhibitor cocktail (Set III, Calbiochem). .. Cleaved caspase-3 and p-gamma-H2AX were assessed by immunoblot and imaged on a Bio-Rad VersaDoc imaging system (CHLA-225, COG-N-269, and COG-N-452h) or using Amersham Hyperfilm ECL (COG-N-561h). .. Anti-cleaved caspase-3 (Cell Signaling, 9664S) and anti-phosphoserine-139 gamma-H2AX (Novus, NB100–384) antibodies were diluted 1:1000.

    Article Title: TPPU inhibits inflammation-induced excessive autophagy to restore the osteogenic differentiation potential of stem cells and improves alveolar ridge preservation.
    Article Snippet: After washing, membranes were incubated for 1 h with the secondary horseradish peroxidase antibody (Solarbio, Beijing, China). .. Finally, the intensity of the bands was determined using the Bio-Rad VersaDoc imaging system and the ECL kit (Solarbio, Beijing, China). ..

    Article Title: CD38–RyR2 axis–mediated signaling impedes CD8 + T cell response to anti-PD1 therapy in cancer
    Article Snippet: Concurrently, pre-stained protein markers (Invitrogen, Thermo Fisher Scientific, Waltham, MA) were run in parallel to identify the molecular weight of the detected proteins. .. Chemiluminescent detection with Clarity Western ECL substrate (Bio-Rad, Hercules, CA) was visualized on Bio-Rad Versadoc Imaging System. .. The same membrane was then stripped and reprobed with β-actin antibody (Cell Signaling Technologies, Danvers, MA) for normalization.

    Article Title: ATG2A engages Rab1a and ARFGAP1 positive membranes during autophagosome biogenesis
    Article Snippet: The membranes were blocked with 5% BSA (Sigma-Aldrich, A9647) in PBS-T (PBS containing 0.1% Tween 20; Thermo Scientific, CAS 9005-64-5) for 1 h at room temperature and incubated with primary antibody (diluted 1:1,000 in PBS-T containing 5% BSA [Sigma-Aldrich, A9647] and 0.03% sodium azide (Sigma-Aldrich, S2002)) overnight at 4°C. .. Membranes were washed three times in PBS-T before incubation with the secondary antibody which was diluted 1:5,000 in PBS-T containing 0.5% Omniblock (American Bio, AB10109-00100) for 1 h. Membranes were then washed three times in PBST and treated with SuperSignal West Femto substrate (Thermo Fisher Scientific, 34096) for 5 min before imaging with the Bio-Rad VersaDoc imaging system. ..

    Article Title: Protein Kinase C Alpha Cellular Distribution, Activity, and Proximity with Lamin A/C in Striated Muscle Laminopathies
    Article Snippet: To remove previous antibody probing(s), membranes were stripped using 1× stripping buffer (Millipore Sigma Canada). .. The ECL detection reagent kit (Amersham (under Cytiva), Mississauga, ON, Canada) was used to visualize the bands via the Bio-Rad VersaDoc Imaging System or the Bio-Rad ChemiDocTM Touch. .. Raw membrane images were analyzed using Bio-Rad Image Lab 6.0.1 (software v 2.0.0.25).

    Article Title: Leu33Pro (PlA) polymorphism of integrin beta3 modulates platelet Src pY418 and focal adhesion kinase pY397 phosphorylation in response to abnormally high shear stress
    Article Snippet: Objectives Shear stress can activate platelet integrinmediated signaling that leads to shear-induced platelet aggregation (SIPA) and eventually contribute to acute myocardial infarction.. The major platelet integrin aIIbb3 is polymorphic at residue 33 [Leu33Pro (PlA) polymorphism].. The Pro33 isoform has been shown to have a prothrombotic phenotype.

    Article Title: Different genetic determinants for high virulence, transmission and replication of high pathogenicity H7N7 avian influenza virus in turkeys and chickens
    Article Snippet: Beta-actin (Clone AC74, Sigma-Aldrich) was used as housekeeping protein. .. Peroxidase conjugated chicken-specific rabbit IGY ++ or anti-mice IgG (Dianova, Hamburg, Germany) at a concentration of 1:20000 in TBS-T. Immunodetection was achieved by chemiluminescence using Supersignal West Pico chemiluminescent substrate kit (Pierce, ThermoScientific, Rockford, IL, USA) and images were captured using a Bio-Rad VersaDoc Imaging System and Quantity One software. ..

    Incubation:

    Article Title: ATG2A engages Rab1a and ARFGAP1 positive membranes during autophagosome biogenesis
    Article Snippet: The membranes were blocked with 5% BSA (Sigma-Aldrich, A9647) in PBS-T (PBS containing 0.1% Tween 20; Thermo Scientific, CAS 9005-64-5) for 1 h at room temperature and incubated with primary antibody (diluted 1:1,000 in PBS-T containing 5% BSA [Sigma-Aldrich, A9647] and 0.03% sodium azide (Sigma-Aldrich, S2002)) overnight at 4°C. .. Membranes were washed three times in PBS-T before incubation with the secondary antibody which was diluted 1:5,000 in PBS-T containing 0.5% Omniblock (American Bio, AB10109-00100) for 1 h. Membranes were then washed three times in PBST and treated with SuperSignal West Femto substrate (Thermo Fisher Scientific, 34096) for 5 min before imaging with the Bio-Rad VersaDoc imaging system. ..

    Concentration Assay:

    Article Title: Different genetic determinants for high virulence, transmission and replication of high pathogenicity H7N7 avian influenza virus in turkeys and chickens
    Article Snippet: Beta-actin (Clone AC74, Sigma-Aldrich) was used as housekeeping protein. .. Peroxidase conjugated chicken-specific rabbit IGY ++ or anti-mice IgG (Dianova, Hamburg, Germany) at a concentration of 1:20000 in TBS-T. Immunodetection was achieved by chemiluminescence using Supersignal West Pico chemiluminescent substrate kit (Pierce, ThermoScientific, Rockford, IL, USA) and images were captured using a Bio-Rad VersaDoc Imaging System and Quantity One software. ..

    Immunodetection:

    Article Title: Different genetic determinants for high virulence, transmission and replication of high pathogenicity H7N7 avian influenza virus in turkeys and chickens
    Article Snippet: Beta-actin (Clone AC74, Sigma-Aldrich) was used as housekeeping protein. .. Peroxidase conjugated chicken-specific rabbit IGY ++ or anti-mice IgG (Dianova, Hamburg, Germany) at a concentration of 1:20000 in TBS-T. Immunodetection was achieved by chemiluminescence using Supersignal West Pico chemiluminescent substrate kit (Pierce, ThermoScientific, Rockford, IL, USA) and images were captured using a Bio-Rad VersaDoc Imaging System and Quantity One software. ..

    Software:

    Article Title: Different genetic determinants for high virulence, transmission and replication of high pathogenicity H7N7 avian influenza virus in turkeys and chickens
    Article Snippet: Beta-actin (Clone AC74, Sigma-Aldrich) was used as housekeeping protein. .. Peroxidase conjugated chicken-specific rabbit IGY ++ or anti-mice IgG (Dianova, Hamburg, Germany) at a concentration of 1:20000 in TBS-T. Immunodetection was achieved by chemiluminescence using Supersignal West Pico chemiluminescent substrate kit (Pierce, ThermoScientific, Rockford, IL, USA) and images were captured using a Bio-Rad VersaDoc Imaging System and Quantity One software. ..



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